Silencing of the PiAvr3a effector-encoding gene from Phytophthora infestans by transcriptional fusion to a short interspersed element.
Identifieur interne : 001660 ( Main/Exploration ); précédent : 001659; suivant : 001661Silencing of the PiAvr3a effector-encoding gene from Phytophthora infestans by transcriptional fusion to a short interspersed element.
Auteurs : Ramesh R. Vetukuri [Suède] ; Zhendong Tian ; Anna O. Avrova ; Eugene I. Savenkov ; Christina Dixelius ; Stephen C. WhissonSource :
- Fungal biology [ 1878-6146 ] ; 2011.
Descripteurs français
- KwdFr :
- Extinction de l'expression des gènes (MeSH), Facteurs de virulence (génétique), Facteurs de virulence (métabolisme), Génétique inverse (méthodes), Maladies des plantes (parasitologie), Phytophthora infestans (génétique), Phytophthora infestans (métabolisme), Phytophthora infestans (pathogénicité), Solanum tuberosum (parasitologie), Transcription génétique (MeSH), Virulence (MeSH), Éléments SINE (MeSH).
- MESH :
- génétique : Facteurs de virulence, Phytophthora infestans.
- métabolisme : Facteurs de virulence, Phytophthora infestans.
- méthodes : Génétique inverse.
- parasitologie : Maladies des plantes, Solanum tuberosum.
- pathogénicité : Phytophthora infestans.
- Extinction de l'expression des gènes, Transcription génétique, Virulence, Éléments SINE.
English descriptors
- KwdEn :
- Gene Silencing (MeSH), Phytophthora infestans (genetics), Phytophthora infestans (metabolism), Phytophthora infestans (pathogenicity), Plant Diseases (parasitology), Reverse Genetics (methods), Short Interspersed Nucleotide Elements (MeSH), Solanum tuberosum (parasitology), Transcription, Genetic (MeSH), Virulence (MeSH), Virulence Factors (genetics), Virulence Factors (metabolism).
- MESH :
- chemical , genetics : Virulence Factors.
- genetics : Phytophthora infestans.
- metabolism : Phytophthora infestans, Virulence Factors.
- methods : Reverse Genetics.
- parasitology : Plant Diseases, Solanum tuberosum.
- pathogenicity : Phytophthora infestans.
- Gene Silencing, Short Interspersed Nucleotide Elements, Transcription, Genetic, Virulence.
Abstract
Phytophthora infestans is the notorious oomycete causing late blight of potato and tomato. A large proportion of the P. infestans genome is composed of transposable elements, the activity of which may be controlled by RNA silencing. Accumulation of small RNAs is one of the hallmarks of RNA silencing. Here we demonstrate the presence of small RNAs corresponding to the sequence of a short interspersed retrotransposable element (SINE) suggesting that small RNAs might be involved in silencing of SINEs in P. infestans. This notion was exploited to develop novel tools for gene silencing in P. infestans by engineering transcriptional fusions of the PiAvr3a gene, encoding an RXLR avirulence effector, to the infSINEm retroelement. Transgenic P. infestans lines expressing either 5'-infSINEm::PiAvr3a-3' or 5'-PiAvr3a::SINEm-3' chimeric transcripts initially exhibited partial silencing of PiAvr3a. Over time, PiAvr3a either recovered wild type transcript levels in some lines, or became fully silenced in others. Introduction of an inverted repeat construct was also successful in yielding P. infestans transgenic lines silenced for PiAvr3a. In contrast, constructs expressing antisense or aberrant RNA transcripts failed to initiate silencing of PiAvr3a. Lines exhibiting the most effective silencing of PiAvr3a were either weakly or non-pathogenic on susceptible potato cv. Bintje. This study expands the repertoire of reverse genetics tools available for P. infestans research, and provides insights into a possible mode of variation in effector expression through spread of silencing from adjacent retroelements.
DOI: 10.1016/j.funbio.2011.08.007
PubMed: 22115441
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Savenkov</name></author><author><name sortKey="Dixelius, Christina" sort="Dixelius, Christina" uniqKey="Dixelius C" first="Christina" last="Dixelius">Christina Dixelius</name></author><author><name sortKey="Whisson, Stephen C" sort="Whisson, Stephen C" uniqKey="Whisson S" first="Stephen C" last="Whisson">Stephen C. Whisson</name></author></analytic><series><title level="j">Fungal biology</title><idno type="ISSN">1878-6146</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Gene Silencing (MeSH)</term><term>Phytophthora infestans (genetics)</term><term>Phytophthora infestans (metabolism)</term><term>Phytophthora infestans (pathogenicity)</term><term>Plant Diseases (parasitology)</term><term>Reverse Genetics (methods)</term><term>Short Interspersed Nucleotide Elements (MeSH)</term><term>Solanum tuberosum (parasitology)</term><term>Transcription, Genetic (MeSH)</term><term>Virulence (MeSH)</term><term>Virulence Factors (genetics)</term><term>Virulence Factors (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Extinction de l'expression des gènes (MeSH)</term><term>Facteurs de virulence (génétique)</term><term>Facteurs de virulence (métabolisme)</term><term>Génétique inverse (méthodes)</term><term>Maladies des plantes (parasitologie)</term><term>Phytophthora infestans (génétique)</term><term>Phytophthora infestans (métabolisme)</term><term>Phytophthora infestans (pathogénicité)</term><term>Solanum tuberosum (parasitologie)</term><term>Transcription génétique (MeSH)</term><term>Virulence (MeSH)</term><term>Éléments SINE (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Virulence Factors</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Facteurs de virulence</term><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phytophthora infestans</term><term>Virulence Factors</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Genetics</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Facteurs de virulence</term><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Génétique inverse</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Maladies des plantes</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Plant Diseases</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Silencing</term><term>Short Interspersed Nucleotide Elements</term><term>Transcription, Genetic</term><term>Virulence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Extinction de l'expression des gènes</term><term>Transcription génétique</term><term>Virulence</term><term>Éléments SINE</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phytophthora infestans is the notorious oomycete causing late blight of potato and tomato. A large proportion of the P. infestans genome is composed of transposable elements, the activity of which may be controlled by RNA silencing. Accumulation of small RNAs is one of the hallmarks of RNA silencing. Here we demonstrate the presence of small RNAs corresponding to the sequence of a short interspersed retrotransposable element (SINE) suggesting that small RNAs might be involved in silencing of SINEs in P. infestans. This notion was exploited to develop novel tools for gene silencing in P. infestans by engineering transcriptional fusions of the PiAvr3a gene, encoding an RXLR avirulence effector, to the infSINEm retroelement. Transgenic P. infestans lines expressing either 5'-infSINEm::PiAvr3a-3' or 5'-PiAvr3a::SINEm-3' chimeric transcripts initially exhibited partial silencing of PiAvr3a. Over time, PiAvr3a either recovered wild type transcript levels in some lines, or became fully silenced in others. Introduction of an inverted repeat construct was also successful in yielding P. infestans transgenic lines silenced for PiAvr3a. In contrast, constructs expressing antisense or aberrant RNA transcripts failed to initiate silencing of PiAvr3a. Lines exhibiting the most effective silencing of PiAvr3a were either weakly or non-pathogenic on susceptible potato cv. Bintje. This study expands the repertoire of reverse genetics tools available for P. infestans research, and provides insights into a possible mode of variation in effector expression through spread of silencing from adjacent retroelements.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22115441</PMID><DateCompleted><Year>2012</Year><Month>03</Month><Day>02</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1878-6146</ISSN><JournalIssue CitedMedium="Internet"><Volume>115</Volume><Issue>12</Issue><PubDate><Year>2011</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Fungal biology</Title><ISOAbbreviation>Fungal Biol</ISOAbbreviation></Journal><ArticleTitle>Silencing of the PiAvr3a effector-encoding gene from Phytophthora infestans by transcriptional fusion to a short interspersed element.</ArticleTitle><Pagination><MedlinePgn>1225-33</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.funbio.2011.08.007</ELocationID><Abstract><AbstractText>Phytophthora infestans is the notorious oomycete causing late blight of potato and tomato. A large proportion of the P. infestans genome is composed of transposable elements, the activity of which may be controlled by RNA silencing. Accumulation of small RNAs is one of the hallmarks of RNA silencing. Here we demonstrate the presence of small RNAs corresponding to the sequence of a short interspersed retrotransposable element (SINE) suggesting that small RNAs might be involved in silencing of SINEs in P. infestans. This notion was exploited to develop novel tools for gene silencing in P. infestans by engineering transcriptional fusions of the PiAvr3a gene, encoding an RXLR avirulence effector, to the infSINEm retroelement. Transgenic P. infestans lines expressing either 5'-infSINEm::PiAvr3a-3' or 5'-PiAvr3a::SINEm-3' chimeric transcripts initially exhibited partial silencing of PiAvr3a. Over time, PiAvr3a either recovered wild type transcript levels in some lines, or became fully silenced in others. Introduction of an inverted repeat construct was also successful in yielding P. infestans transgenic lines silenced for PiAvr3a. In contrast, constructs expressing antisense or aberrant RNA transcripts failed to initiate silencing of PiAvr3a. Lines exhibiting the most effective silencing of PiAvr3a were either weakly or non-pathogenic on susceptible potato cv. Bintje. This study expands the repertoire of reverse genetics tools available for P. infestans research, and provides insights into a possible mode of variation in effector expression through spread of silencing from adjacent retroelements.</AbstractText><CopyrightInformation>Crown Copyright © 2011. Published by Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vetukuri</LastName><ForeName>Ramesh R</ForeName><Initials>RR</Initials><AffiliationInfo><Affiliation>Department of Plant Biology and Forest Genetics, Uppsala BioCenter, SLU, 750 07 Uppsala, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tian</LastName><ForeName>Zhendong</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Avrova</LastName><ForeName>Anna O</ForeName><Initials>AO</Initials></Author><Author ValidYN="Y"><LastName>Savenkov</LastName><ForeName>Eugene I</ForeName><Initials>EI</Initials></Author><Author ValidYN="Y"><LastName>Dixelius</LastName><ForeName>Christina</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Whisson</LastName><ForeName>Stephen C</ForeName><Initials>SC</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><Agency>Biotechnology and Biological Sciences Research Council</Agency><Country>United Kingdom</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>09</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Fungal Biol</MedlineTA><NlmUniqueID>101524465</NlmUniqueID></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C562170">AVR3a protein, Phytophthora infestans</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D037521">Virulence Factors</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020868" MajorTopicYN="Y">Gene Silencing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059386" MajorTopicYN="N">Reverse Genetics</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020086" MajorTopicYN="Y">Short Interspersed Nucleotide Elements</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011198" MajorTopicYN="N">Solanum tuberosum</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D037521" MajorTopicYN="N">Virulence Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>06</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>08</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>11</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>11</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>3</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22115441</ArticleId><ArticleId IdType="pii">S1878-6146(11)00159-0</ArticleId><ArticleId IdType="doi">10.1016/j.funbio.2011.08.007</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country></list><tree><noCountry><name sortKey="Avrova, Anna O" sort="Avrova, Anna O" uniqKey="Avrova A" first="Anna O" last="Avrova">Anna O. Avrova</name><name sortKey="Dixelius, Christina" sort="Dixelius, Christina" uniqKey="Dixelius C" first="Christina" last="Dixelius">Christina Dixelius</name><name sortKey="Savenkov, Eugene I" sort="Savenkov, Eugene I" uniqKey="Savenkov E" first="Eugene I" last="Savenkov">Eugene I. Savenkov</name><name sortKey="Tian, Zhendong" sort="Tian, Zhendong" uniqKey="Tian Z" first="Zhendong" last="Tian">Zhendong Tian</name><name sortKey="Whisson, Stephen C" sort="Whisson, Stephen C" uniqKey="Whisson S" first="Stephen C" last="Whisson">Stephen C. Whisson</name></noCountry><country name="Suède"><noRegion><name sortKey="Vetukuri, Ramesh R" sort="Vetukuri, Ramesh R" uniqKey="Vetukuri R" first="Ramesh R" last="Vetukuri">Ramesh R. Vetukuri</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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